Figure 3.

Genetic Interactions of MGO1 and WUS.

(A) to (E) Eleven-day-old seedlings. In wild-type (A), mgo 1-4 (B), and wus-7 (D) seedlings, the first leaves have been formed. In the severe wus-1 mutant (C), intermediate wus-6 mutant (inset), and in the mgo 1-4 wus-7 double mutant (E), the primary shoot meristem has terminated without leaf formation.

(F) and (G) Comparison of wus-1 (F) and wus-6 (G) 45-d-old plants. Adventitious shoots in wus-1 terminated prematurely in an aerial rosette, whereas wus-6 produces an indeterminate shoot with many flowers.

(H) wus-6 mgo1-4 double mutants rarely formed adventitious shoots and never produced flowers.

(I) Comparison of wus-1, wus-7, and mgo 1-4 wus-7 40-d-old plants. mgo1-4 wus-7 double mutants formed determinate adventitious shoots with a few defective flowers similar to wus-1, whereas wus-7 single mutants produced indeterminate shoot with many flowers.

(J) to (M) Flower phenotype of wus-1 (J), wus-6 (K), wus-7 (L), and mgo1-4 wus-7 (M) plants.

(N) Quantitative RT-PCR experiment showing mRNA expression levels of WUS. Asterisk represents significant difference of WUS mRNA compared with the wild type (P < 0.05).

(O) No changes in MGO1 transcript levels are detectable by RT-PCR after induction of 35S:WUS-GR plants with dexamethasone (D) compared with mock treated plants (M). ACT7 (actin7) transcript was used as control.

Bars = 2.5 mm in (A) to (E), 2 cm in (F) to (H), 1 cm in (I), and 2 mm in (J) to (M).