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. 2010 Mar 26;22(3):850–866. doi: 10.1105/tpc.109.073270

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© 2010 American Society of Plant Biologists

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Figure 5. — HPLC-MS Analysis of the Products of GT3 Activity with a Crude Sapogenin Extract from Medicago Roots.

(A) Selected base peak chromatogram of the crude sapogenin extract (blue) and glucosylated products (red).

(B) Structure of hederagenin 28-O-β-d-glucopyranoside.

(C) to (H) Negative-ion HPLC-ESI-MS/MS of sapogenins and their glucosides produced by the action of UGT73F3 on a crude sapogenin extract from M. truncatula: bayogenin glucoside (C), unidentified A glucoside (D), unidentified B glucoside (E), hederagenin glucoside (F), medicagenic acid aglycone (G), and medicagenic acid glucoside (H). Explanation of masses for molecules indicated in square brackets: M, molecule; Glc, β-d-glucopyranosyl; Hac, acetic acid; Na, sodium; and H, hydrogen.