Figure 7.

Phenotypic Analysis of Δexp5.

(A) Colony morphology and growth chart on CM and MM of the wild type, Δexp5, and Δexp5 complemented strains with amino (Δexp5/EXP5-mRFP-N) and carboxy (Δexp5/EXP5-mRFP-C) protein fusions. Error bars represent the sd.

(B) Appressorium development assays of M. oryzae strains on PHOB-PS at 4, 6, and 8 h. Bar = 20 μm.

(C) Leaf and root blast symptoms (4 and 15 d after inoculation, respectively) of M. oryzae wild-type, Δexp5, and Δexp5/ EXP5-mRFP complemented strains.

(D) Cellular localization of EXP5-mRFP protein during conidial germination on rice leaf sheath and rice roots. Bar = 10 μm.