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. Author manuscript; available in PMC: 2010 Dec 10.
Published in final edited form as: J Neuroimmunol. 2009 Oct 30;217(1-2):65–73. doi: 10.1016/j.jneuroim.2009.10.007

FIGURE 3.

FIGURE 3

Anti-DOR autoantibodies induce phosphorylation of DOR at Ser 363. CEM×174 cells were incubated with 10 μM DPDPE or 100μg anti-DOR Ab for 10 And 30 min in serum-free media. Cells were lysed and 10 μg of total protein was run on 10% denaturing SDS-PAGE gels and transferred to a PVDF membrane. Western blot analysis was performed using an anti-phospho Ser 363 DOR antibody. Anti-DOR autoantibodies cause significant phosphorylation of the receptor at 10 min and 30 min. DPDPE causes phosphorylation of DOR as early as 10 min that decreases significantly by 30 min.