Autoantibodies to DOR loops 1 and 3 are present in the sera of healthy
individuals, SLE and HIV patients. Plates were coated with peptides
corresponding to DOR and MOR loop 1 (L1) and 3 (L3) peptides. We tested the
serum of 4 healthy individuals (N), 7 SLE patients and 13 HIV patients.
Values reflect mean absorbance at 450nm ± s.d. of serum samples
diluted 1 in 400. SLE serum samples show significantly lower binding to DOR
loops 1 and 3 than normal sera (A, p<0.05). The converse is seen
in binding to MOR loops, where SLE sera show higher binding than normal sera
(B). HIV sera show significantly lower binding to DOR loops 1 and 3 than
normal sera (C, p<0.001). The trend is reversed in binding to MOR
loops 1 and 3 (D). Statistical significance was determined with a one-way
analysis of variance, Bonferroni’s post-test was used to obtain
p-values for the indicated pairwise comparisons.