FIG. 3.

Role of dynamin and lipid rafts in TNF-α and thrombin generation of ROS. SMCs expressing dn-dynamin were incubated with DCFH and fluorescence measured in response to TNF-α or thrombin by (A) microscopy or by (B) flow cytometry (n = 5). Control represents cells infected with AdshGFP. (C) SMCs were treated with 5 mM MBCD for 30 min prior to TNF-α or thrombin and DCF fluorescence measured by flow cytometry. (n = 4). *p < 0.05 vs. control for respective agonist. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article at www.liebertonline.com/ars).