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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Cell Immunol. 2009 Dec 5;261(2):153–158. doi: 10.1016/j.cellimm.2009.12.002

Fig. 1.

Fig. 1

Trojan peptide constructs. Trojan peptides contained the cell-permeable penetratin sequence of Antennapedia in tandem with the C-terminal end of FcγRIIA’s cytoplasmic domain. The penetratin sequence is underlined, and the Fc receptor sequence is in bold type with its critical motif highlighted. As a control, a peptide was constructed in which the LTL motif was substituted with AAA. The peptide containing the LTL motif (Trojan-LTL peptide) was conjugated to tetramethylrhodamine whereas the AAA substituted (Trojan-AAA) peptide was labeled with fluorescein. Prior to use, the lyophilized peptides were dissolved in a stock solution with sterile PBS at a concentration of 900 μM, and stored at −20° C. Prior to use peptides were diluted to a concentration of 45 μM.