Fig. 2.

The Trojan-LTL peptide labels an ER-like cellular compartment. Adherent neutrophils were labeled with Trojan-LTL peptide, the control Trojan-AAA peptide, or ER-Tracker for 1 hour at 37°C and imaged as described in Materials and Methods. The Trojan-LTL peptide accumulated within cells (A and B). This pattern of labeling was very similar to the labeling pattern of cells stained with ER Tracker (C and D), suggesting that Trojan-LTL peptides may interact with the ER. In cells labeled with the Trojan-AAA peptide only a diffuse non-specific labeling pattern was seen throughout the cell (E and F), suggesting that Trojan-AAA peptides enter cells, but do not selectively bind to organelles. (x1800) (A and B, n=6; C and D, n=5; E and F, n=3)