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. Author manuscript; available in PMC: 2010 Apr 29.
Published in final edited form as: Free Radic Biol Med. 2008 Jan 18;44(8):1570–1577. doi: 10.1016/j.freeradbiomed.2008.01.003

Fig. 5.

Fig. 5

Kinetics of absorbance changes at 432 nm in the absence and presence of saturated amounts of Trp as measured by stopped-flow spectroscopy at 10 °C. One syringe contained MPO-Fe(III) (μM) (left trace) or MPO pre-incubated with saturated amounts of Trp (400 μM) (right trace) and was rapidly mixed with a syringe containing 40 μM H2O2. Note that in the absence of Trp, the change in absorbance was attributed to the formation of MPO Compound I. In the presence of Trp, the change in absorbance was attributed to the direct conversion of MPO-Fe(III) to MPO Compound II. The smooth line running through each experimental trace is the line of best fit according to a one exponential equation.