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. Author manuscript; available in PMC: 2010 Apr 29.
Published in final edited form as: Free Radic Biol Med. 2008 Jan 18;44(8):1570–1577. doi: 10.1016/j.freeradbiomed.2008.01.003

Fig. 6.

Fig. 6

Trp competes with plasma level of Cl- and switches the MPO reaction from a 2e− oxidation to a 1e− oxidation pathway. Absorbance spectra of MPO recorded by diode array stopped-flow when a phosphate buffer (200 mM, pH 7.0) containing 1.9 μM MPO, 200 mM Cl−, and 400 μM Trp was rapidly mixed with a buffer solution supplemented with 40 μM H2O2 at 10 °C. This figure contains spectra collected at 0.001, 0.016 0.031, 0.073, 0.103, 0.180, 0.240 and 1.425 s after initiating the reaction. Arrows indicate the direction of spectral change over time.