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. 2010 Apr 29;5(4):e10409. doi: 10.1371/journal.pone.0010409

Figure 2. Tenascin-C upregulates Beclin 1 through 14-3-3τ.

Figure 2

(A–B) MCF7 cells were grown on Fibronectin (FN) or Tenascin C (TN) pre-treated plates. Next day, cells were infected with Adsi14-3-3τ or control AdsiScr virus at MOI 100 for 48 hrs. Cells were then harvested for Western blot (A, upper panels), semiquantitative RT-PCR (A, lower panels), and real-time RT-qPCR analyses (B). No RNA lane represents a control RT-PCR reaction without input RNA. Quantitative PCR was performed in triplicate. The levels of Beclin 1 were normalized to GAPDH levels and are expressed relative to the expression of the gene in the siScr FN control. The data shown represent the means ± standard deviations of three independent samples. The p values are based on a paired two-tailed t test. (C–D) U2OS cells were infected by Ad14-3-3τ (Ad14) or an empty vector AdCMV (CMV) at MOI 300 for 2 days. Protein and RNA were harvested for Western blot analysis (C) or real-time RT-qPCR (D). Quantitative PCR was performed in triplicate. The levels of Beclin 1 were normalized to GAPDH levels and expressed relative to the expression of the gene in the AdCMV control. The data shown represent the means ± standard deviations of three independent samples. The p values are based on a paired two-tailed t test.