Figure 8.

Anillin and DE-cadherin restrict F-actin to the cleavage furrow. (A–D) Time-lapse images showing the change in localization of mCherry-Utr-CH (which binds F-actin) during spermatocyte cytokinesis. In control cells (A) and cells expressing DE-cad-GFP (B), mCherry-Utr-CH is tightly associated with the furrow, whereas in dsRNA-expressing cells mCherry-Utr-CH moves around the cell cortex in an oscillatory manner (C). DE-cad-GFP expression stabilizes mCherry-Utr-CH at the furrow in anillin-depleted cells (D). The images shown were taken at 40-s intervals. Corresponding full-length videos are available online (Supplemental Videos 3–6). Bar, 5 μm.