Figure 2.

A network of direct protein-protein interactions linking the GPR loop of MyoK, Abp1, profilins, and dynamin A was revealed by blot overlays. GST-Abp1 directly interacts with immobilized GPR loop (A) and dynamin A (B). Purified profilin I and II were overlaid on membranes blotted in parallel (C). Both isoforms clearly interact with immobilized Abp1 and dynamin A. Weak interaction of profilin II with Abp1 and profilin I with WASp PRD are detected although the signal intensity of the control (GST) is similar. A Ponceau staining is shown (left) and corresponding overlays are shown (right). GST and the MyoD head domain were used as negative controls for GST fusion or His-tagged proteins, respectively.