Figure 1.

Live cell microscopy shows that Sec13p-GFP and Sec23p-GFP localize to tER sites on the ER membrane. (A) Still images of wild-type strains expressing Sec63-mRFP (CBY2716), Sec13p-GFP (CBY1829), and Sec23p-GFP (CBY2712). Note that both Sec13p-GFP and Sec23p-GFP punctae localize to the ER vicinity, outlined in the cell periphery and perinuclear region by Sec63p-mRFP. (B) A wild-type strain (CBY2969), expressing both Sec13p-GFP and Sec23p-GFP, was imaged in the GFP and the RFP channels. Note significant overlap between ER and Sec13p-GFP punctae. (C) Still images from a video of cells expressing Sec13p-GFP (CBY1829), acquired by four-dimensional spinning disk confocal microscopy. Numbers indicate the time in seconds from start of acquisition. Arrowheads indicate the position of tER sites that could be tracked through each of the video frames. Bars, 5 μm.