Figure 2.

Vesicle-budding mutant sec12-4 cells, but not wild-type or fusion mutant uso1-1 cells, exhibit altered tER site morphology at a restrictive temperature. (A) Wild-type (CBY1829), sec12-4 (CBY1772) and uso1-1 (CBY2013) strains were grown at 22°C, imaged, and then shifted to 37°C. Images were acquired at 0, 15, and 120 min after the shift. (B) Quantification of total fluorescence intensities of individual tER sites from the images obtained in A. All tER sites were analyzed in 10 randomly selected cells per condition. Points on the plot indicate individual fluorescence intensity values. (C) Quantification of the percentage of cell populations that showed altered tER site morphology, from the images obtained in A. Forty randomly selected cells were analyzed per condition.