Table 2.
Summary of tER morphologies observed in temperature-sensitive mutants
| Function | Mutation | Sec13p-GFPdistribution | Sec23p-GFPdistribution |
|---|---|---|---|
| Vesicle budding | sec12-4 | Large punctaa | Large puncta |
| sec16-2 | Large puncta | Large puncta | |
| sec23-1 | Alteredb | nd | |
| sec24-11 | wt | Altered | |
| sec13-1 | nd | Altered | |
| sec31-1 | wt | wt | |
| yip1-4 | wt | wt | |
| Vesicle fusion | uso1-1 | wt | wt |
| sed5-1 | wt | wt | |
| sec18-1 | wt | wt | |
| ypt1-3 | wt | nd |
nd, not determined; wt, wild type.
a Large puncta, >50% of cells accumulate one to two large bright puncta.
b Altered, >50% of cells display one to two brighter puncta and/or a diffuse GFP pattern.