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. 2010 May 1;21(9):1556–1568. doi: 10.1091/mbc.E09-12-1002

Figure 4.

Figure 4.

The whorled ER phenotype is rescued by a wild type but not mutant siRNA-immune Yip1A construct. Cells cotransfected with Yip1A siRNA-2 and either a control Sec13-Myc construct (A and B), a siRNA-immune wild-type FLAG-Yip1A construct (C and D), or a siRNA-immune mutant (E95K) FLAG-Yip1A construct (E and F) were fixed 72 h later and doubly stained with antibodies against the Myc epitope (A) and calnexin (B) or the FLAG epitope (C and E) and calnexin (D and F). Single asterisks (A–F) indicate expressing cells that exhibit ER whorls. Double asterisks (A–F) indicate expressing cells that do not exhibit ER whorls. Bar, 10 μm. (G) Quantitation of the percentage of Sec13-Myc or wild type or mutant (E95K) FLAG-Yip1A–expressing cells displaying the whorled ER phenotype from three independent experiments (>50 cells per experiment), ±SD. Single asterisks indicate p < 0.0001 (Student's t test). Double asterisk indicates no statistically significant difference.