Table 2. Clinical manifestation in swine inoculated with C-S8p260 and challenged with FMDV C-S8c1.

Group	Inoculum(PFUs of C-S8p260)	Animal	Viremia (dpc, day of onset, duration in days)a	Nasal swab specimen (dpc, day of onset, duration [days])b	No. of lesions (day of onset)c
1	5×106+Adj	1	Neg.	Neg.	Neg.
		2	Neg.	Neg.	Neg.
		3	Neg.	Neg.	Neg.
		4	Neg.	Neg.	Neg.
		5	Neg.	Neg.	Neg.
		6	2.5×105 (4, 4, 1) [Neg.]	Neg.	Neg.
2	105+Adj	7	Neg.	Neg.	Neg.
		8	Neg.	Neg.	Neg.
		9	Neg.	Neg.	Neg.
3	5×106	10	2.5× 105 (2, 2, 2) [Neg.]	Neg	Neg
		11	3.1×105 (2, 2, 2) [Neg.]	Neg	Neg
4	BEI inactivated	12	7×105 (2, 2, 4) [1.2×104]	2.8×105 (4, 4, 1)	5 (3)
		13	2×105 (2, 2, 4) [1×104]	3.7×106 (4, 4, 1)	5 (4)
		14	Neg.	Neg.	Neg.
5	Non vaccinated	15	6.2×109 (3, 1, 5) [1×105]	2×108 (4, 2, 2)	15 (2)
		16	7×109 (3, 1, 5) [1.1×105]	1.2×107 (4, 3, 2)	16 (2)
		17	1.1×109 (3, 1, 6) [6.5×104]	1.1×106 (4, 3, 2)	11 (2)

^aViremia is expressed as FMDV RNA molecules/ml of serum quantified by ^qRT-PCR (Neg. means<10³ FMDV RNA molecules/ml of serum). The three numbers given in parenthesis correspond to: dpc, the day after challenge at which the maximum level of viremia was detected; the first dpc at which viremia was detected; and the third value is the number of days of duration of viremia. The infectivity of those sera that were positive for FMDV RNA is indicated in brackets as PFU/ml of serum; [Neg.] means<100 PFU/ml of serum.

^bFMDV RNA molecules per ml of nasal secretion (Neg. means<10³ FMDV RNA molecules per ml of nasal secretion). The dpc, onset, and duration values are as defined in footnote a.

^cNumber of toes with lesions plus the snout and tongue combined, when lesions were present. The maximum score is 17. The day of onset (given in parenthesis) is the first day after challenge at which lesions were detected.