Figure 5. Role of PS-exposing platelets in fibrin formation during thrombus formation.

Thrombi were formed on fibrinogen by co-perfusion of whole blood and CaCl₂/TF at a shear rate of 200 s⁻¹. Blood samples were incubated with vehicle, annexin A5, or mutant M1234 annexin A5 (each 0.28 µmol/L) before perfusion. Thrombi were labeled with OG488-fibrinogen (0.3 µmol/L). A, Representative phase-contrast micrographs of platelets and fibrin after 15 minutes of perfusion. B, Relative accumulation of labeled fibrin(ogen). Data are presented as integrated fluorescence intensity per microscopic field. Results of one representative experiment are shown.