Figure 6. Incorporation of labeled coagulation factors during venous thrombus formation in vivo.

Mice were injected with OG488-labeled fibrinogen (FG), prothrombin (Prothr), factor Xa (FXa), or anti-CD41 mAb (anti-platelet integrin α_IIb). Thrombus formation in exteriorized mesenteric vessels was induced by 30 µL of 500 mM FeCl₃ (n = 3–5). A, Fluorescence images of venous thrombi (arrows) and adjacent arteries (arrowheads). B, Increase in fluorescence surface area coverage over time. C, Distribution of fluorescence in smaller or larger features.