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Effect of juvenile hormone on the expression of the four hexamerin genes. The hormone (diluted in acetone) was topically applied on the dorsum of 5th instar larvae (feeding phase, L5F). Controls were treated with acetone only. Gene expression was analyzed 24 h after treatment. Hexamerin transcript abundance analyzed by RT-PCR followed by electrophoresis of the amplified cDNA on ethidium bromide-stained agarose gels. The A. mellifera rp49 gene was used as a loading control.
