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. 2010 Apr 29;5(4):e10428. doi: 10.1371/journal.pone.0010428

Figure 4. Cytoplasmic but not nuclear ICP0 inhibits IRF3 activation and subsequent ISG induction.

Figure 4

(A) HEL cells were infected with WT HSV-1 (strain F), ICP0 null HSV-1 (R7910), R7914, or D8 for the indicated times. Cytoplasmic and nuclear protein extracts were resolved using native western blots to examine IRF3 dimerization. (B) HEL cells were infected as denoted in part A. Total and phospho-IRF3 (ser-396) levels were examined by western blotting. (C) ISG56 and ICP0 expression were measured using western blot analysis following an 8 hour infection as indicated. In all cases, SeV served as a positive control for activation of the IRF3 pathway.