Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Apr 29;6(4):e1000873. doi: 10.1371/journal.ppat.1000873

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Bruns et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Lys-EGFP mice were infected and acute lung slices were prepared as described in Figure 4. Subsequently, time-lapse 2-photon microscopy was used to generate movies of cells migrating in these lung slices. (A) A still image of a movie showing individual neutrophils (green), DNA (red) and tissue/fungal elements (blue). Tracks of migrating cells are shown in white. Image of the tracks alone (Video S4). (B) Many neutrophils (green) can be seen migrating within the tissue and internalising conidia in slices. The red square is shown as a magnification on the right (white arrowheads denote phagocytosis events). Tissue (dark blue) fungal elements (light blue), DNA (red, Video S5). (C) A still image from the middle of a Z-stack of an infected lung in a Lys-EGFP animal. The area boxed in white is shown enlarged from the bottom and as 3-D rendering from the side to demonstrate the internalisation of a conidium (light blue) within a neutrophil (green). See also Video S9. (D) Multiple neutrophils (green) cooperate to transport a hypha (White arrowhead, hypha is light blue. The area of the red square is shown as a magnification below.), that is too big to be engulfed, to an area with more neutrophils (Video S6). Red: DNA from nuclei and NETs. Similar events can also be observed in vitro (Video S10). (E) An individual neutrophil (green, arrowhead) enters the alveolar space and migrates along the alveolar surface (Dark blue structure. The white track is the migration path of the neutrophil.). The border of the alveolus is depicted with a broken blue line in the magnification of the area identified by the red square (Video S7). The images are representative of 8 individual mice that were analysed.

Inline graphic — Lys-EGFP mice were infected and acute lung slices were prepared as described in Figure 4. Subsequently, time-lapse 2-photon microscopy was used to generate movies of cells migrating in these lung slices. (A) A still image of a movie showing individual neutrophils (green), DNA (red) and tissue/fungal elements (blue). Tracks of migrating cells are shown in white. Image of the tracks alone (Video S4). (B) Many neutrophils (green) can be seen migrating within the tissue and internalising conidia in slices. The red square is shown as a magnification on the right (white arrowheads denote phagocytosis events). Tissue (dark blue) fungal elements (light blue), DNA (red, Video S5). (C) A still image from the middle of a Z-stack of an infected lung in a Lys-EGFP animal. The area boxed in white is shown enlarged from the bottom and as 3-D rendering from the side to demonstrate the internalisation of a conidium (light blue) within a neutrophil (green). See also Video S9. (D) Multiple neutrophils (green) cooperate to transport a hypha (White arrowhead, hypha is light blue. The area of the red square is shown as a magnification below.), that is too big to be engulfed, to an area with more neutrophils (Video S6). Red: DNA from nuclei and NETs. Similar events can also be observed in vitro (Video S10). (E) An individual neutrophil (green, arrowhead) enters the alveolar space and migrates along the alveolar surface (Dark blue structure. The white track is the migration path of the neutrophil.). The border of the alveolus is depicted with a broken blue line in the magnification of the area identified by the red square (Video S7). The images are representative of 8 individual mice that were analysed.