Figure 2. Hypersensitivity of telomeres relative to coding regions.
The frequency of CPD is ∼7 fold higher in human fibroblast telomeres than in a fragment of the p53 gene or 28S ribosomal DNA at 20 J/m2 UVC. This sensitivity also holds for each telomere strand individually and is unaffected by precise correction for the percentage of dimerizable dipyrimidines in the various sequences. (Upper) Graphical representation of the quantification of the PCR–amplified bands or lanes. The ratio IP/input corrects for copy number. (Lower) Gels of PCR–amplified IP DNA. Each experiment was performed in triplicate. P values are derived from the two-tailed heteroscadastic Student's t-test.