Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Apr 29;6(4):e1000891. doi: 10.1371/journal.ppat.1000891

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

PMC Copyright notice

Complement-opsonization of HIV (A) or AT2-inactivated HIV (B) and subsequent loading of DCs initiates proliferation of CD8⁺ T cells as measured by counting CD8⁺ T cells over a one minute period of time (A) and by CFSE-staining of CD8⁺ T cells (C). As positive control, DCs were exposed to the superantigen SEB, which induced strong proliferation of CD8⁺ T cells. The response to SEB was set at 100%. (A) No or low proliferation comparable to iDC-primed CD8⁺ T cells was mediated by DCs exposed to non-opsonized HIV (HIV). Cytokine-stimulated mDC-primed CD8⁺ T cells showed low proliferation, while HIV-C-exposed DCs induced about 60% proliferation compared to SEB-DC-primed CD8⁺ T cells. Columns represent mean values of CD8⁺ T cells from 5 donors stimulated with either BaL or 92UG037. Data were analyzed by unpaired Student's t-test. (B) Prime-boosting by AT2-HIV-C-loaded DCs caused proliferation of autologous CD8⁺ T cells, albeit weaker than with HIV-C-exposed DCs. In contrast, DCs loaded with AT2-HIV did not induce proliferation of CD8⁺ T cells at all. This was repeated four times with HIV strains 92BR030 or 92UG037. (C) Complement-mediated proliferation of CD8⁺ T cells was confirmed with 5 donors by staining cells with 2.5 µM CFSE prior addition of loaded DCs (n = 5). Left panel: dot plot of CD8⁺ T cells exposed three times to SEB-, HIV- or HIV-C-loaded DCs, and a CFSE⁺ control. The amount of cells in the live CD8^high population (P3) is shown in the dot plot for one representative donor. Right panel: mean percentages of CFSE⁻ [proliferated]/CD8⁺ T cells of SEB-, HIV- or HIV-C-DC-primed CD8⁺ T cells from 5 donors. Data were analyzed by unpaired Student's t-test.