Figure 1.

Adipose-derived stem cell isolation techniques flow chart. The wide variance in the time, materials, and effort for obtaining ASCs via the Standard Isolation and our Rapid Isolation techniques is shown. A highly viable population of around 250,000 ASCs can be derived from 250 ml of blood/saline fraction of the liposuction waste in as little as 30 minutes, as compared to 8–10 hours to obtain ASCs using the traditional isolation method. SVF, stromal vascular fraction; ABAM, antibiotic/antimycotic; FBS, fetal bovine serum.