Fig. 1.

Real-time quantitative RT-PCR detection of TRPC1 and TRPC6 mRNAs isolated from 2- to 4- and 16- to 20-month old rat aorta. PCR products amplified with gene-specific primers (Table 1) for TRPCs and β-actin. mRNA levels were quantified based on their cycle threshold (see “Materials and methods” section for details) and normalized to internal β-actin expression. Values were given as [TRPCx]/[β-actin] × 10⁴ (n = 4–6). Inset image confirms the specificity of the PCR products (TRPC1 410 bp, TRPC6 321 bp, and β-actin 244 bp) run on agarose gel after qRT-PCR. Marker—50-bp DNA Ladder (Fermentas)