Figure 2. Strand specificity of FRT-seq.

Sequences generated by FRT-seq were mapped against the human genome,. .wig files are displayed in IGB, though the colours were modified for clarity (dark red). For comparison, sequences made using the standard RT-seq library preparation protocols and flowcell amplification are also shown (blue). Below is a representation of the region of human chromosome 1p36, and beneath this genes are shown in Ensembl together with the strands from which the transcript is produced.