Figure 5.

Effect of bound EcoRI on the processivity of AAG. Multiple turnover processivity assays were performed with 200 nM 72εA²F², 2 nM full-length AAG, and 0, 210, and 420 nM EcoRI dimer at both 100 and 200 mM ionic strength and the calculated processivity values are shown. The presence of the tightly bound EcoRI dimer reduces the processivity of AAG by ∼50% at ionic strengths of 100 mM (black bars) and 200 mM (gray bars).