. Author manuscript; available in PMC: 2010 Apr 30.

Published in final edited form as: Mol Cell Proteomics. 2007 Oct 19;7(1):35–45. doi: 10.1074/mcp.M700353-MCP200

TABLE 3.

Eight Rsp5 substrates

Substrate	Knockout phenotype^*	Ranking on Chip	PPI^*	GI^**	Ubr1^***	Function
Rpn10	Reduced fitness	9	−	S	−	Ubiquitin-dependent protein catabolism
Rnr2	Inviable	10	−	S	−	DNA replication/ribonucleoside-diphosphate reductase activity
Nsl1	Inviable	31	−	S	−	Chromosome segregation/Essential component of the MIND kinetochore complex
Nkp2	Reduced fitness	12	−	N	+	Non-essential kinetochore protein
Sla1	ts	94	+	W	+	Cytoskeletal protein binding protein
Sla2	ts	5	+	S	−	required for assembly of the cortical actin cytoskeleton
Taf3	Inviable	66	+	W	−	Transcription initiation from RNA polymerase II promoter
Ygr068c	Viable	73	+	S	−	Unknown

Knockout phenotype and PPI (protein protein interaction) data base: YPD and SGD

^**

GI (genetic interaction): dosage lethality/suppression defect induced by over-expression of potential substrates in this study; S indicates strong, W indicates weak, N indicates no genetic interaction.

^***

Ubr1 data is based on protein chip results in this study.