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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Nat Immunol. 2010 Feb 28;11(4):335–343. doi: 10.1038/ni.1847

Figure 8. Rescue of the elektra phenotype by BAC transgenesis.

Figure 8

Transgenic mice were produced by microinjecting a BAC clone containing the wild-type Slfn2 sequence into single-cell embryos homozygous for the elektra mutation. (a) DNA sequence of the BAC clone and genomic DNA from an elektra homozygote in the region of the Slfn2elektra mutation. The BAC clone contains the wild-type Slfn2 sequence. (b) Flow cytometric analysis of CD8 and CD4 staining of blood from a WT mouse, a homozygous elektra transgenic mouse carrying the Slfn2 transgene (elektra-BAC Tg), and a littermate lacking the transgene (elektra). Results are representative of 4 mice per genotype. (c) Survival curves for transgenic mice (elektra-BAC Tg, n=7), littermates without the transgene (elektra, n=4), and WT mice (n=5) upon challenge with 2 × 105 PFU of MCMV. (d) Survival curves for transgenic mice (elektra-BAC Tg, n=7), littermates without the transgene (elektra, n=5), and WT mice (n=7) upon challenge with 5 × 105 CFU of L. monocytogenes.