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. Author manuscript; available in PMC: 2010 Apr 30.
Published in final edited form as: J Chromatogr A. 2008 Dec 6;1216(5):845–856. doi: 10.1016/j.chroma.2008.11.093

Table 2.

Resolution and migration time data gathered from the CCD experiment for the optimization of MEKC parameters.

Exp. # Experimental parameters
 Experimental/model predicted responses
pH [Surfactant] (mM) [NH4OAc] (mM) Voltage (kV) Temperature (°C) Neb. Pres. (psi) Resolution
 Migration timec
BNP BOH (min)
1 11.50 15.0 15.0 20 16.0 3 0.45 0.64 0.70 1.06 6.5 6.8
2 11.50 30.0 15.0 20 16.0 5 0.46 0.52 0.99 0.90 6.9 6.8
3 10.00 30.0 35.0 10 16.0 3 1.24 1.11 0.49 0.54 19.3 18.5
4 11.50 15.0 15.0 10 16.0 3 0.66 0.46 1.11 0.78 12.7 11.8
5 11.50 30.0 35.0 10 25.0 5 0.02 0.11 0.22 0.50 13.9 13.6
6 10.75 22.5 25.0 15 20.5 4 0.82 0.73 1.26 1.11 11.5 10.4
7 11.50 15.0 35.0 20 25.0 3 0.63 0.50 1.61 1.27 8.7 8.3
8 10.75 22.5 25.0 15 20.5 4 0.90 0.73 1.25 1.11 12.1 10.4
9 10.75 22.5 25.0 15 20.5 4 0.89 0.73 1.44 1.11 11.0 10.4
10 11.50 30.0 15.0 20 25.0 3 0.76 0.78 1.22 1.24 7.5 6.9
11 11.50 30.0 15.0 20 25.0 5 0.75 0.45 1.58 0.93 6.9 6.4
12 10.00 30.0 15.0 10 25.0 5 0.75 0.46 0.52 0.40 13.9 11.3
13 11.50 15.0 35.0 20 25.0 5 0.28 0.12 0.65 0.43 7.8 7.0
14 10.00 30.0 35.0 10 25.0 3 1.22 1.15 0.66 0.62 20.3 17.8
15 10.00 30.0 35.0 10 16.0 5 0.69 0.55 0.31 0.39 16.1 15.4
16 11.50 30.0 35.0 10 16.0 5 0.08 0.03 0.62 0.48 14.6 14.2
17 10.00 30.0 35.0 20 16.0 5 0.76 0.51 0.57 0.37 8.6 8.1
18 10.75 22.5 25.0 15 20.5 4 0.80 0.73 1.26 1.11 10.3 10.4
19 10.00 30.0 35.0 20 16.0 3 1.12 1.08 0.78 0.67 9.8 9.8
20 10.00 15.0 15.0 20 25.0 3 0.32 0.19 1.02 0.79 7.1 6.8
21 10.75 22.5 40.7 15 20.5 4 0.77 0.78 1.23 1.24 11.5 12.1
22 10.75 22.5 9.4 15 20.5 4 0.67 0.73 1.22 1.21 8.7 8.7
23 10.00 30.0 35.0 10 25.0 5 0.67 0.61 1.16 0.38 12.1 14.5
24 10.00 15.0 15.0 20 25.0 5 0.20 −0.02 0.25 0.28 5.7 5.9
25 11.50 30.0 15.0 10 25.0 3 0.68 0.62 1.15 0.89 12.1 12.5
26 10.75 10.8 25.0 15 20.5 4 0.32 0.41 1.22 0.96 8.4 9.4
27 10.00 15.0 15.0 10 25.0 5 0.01 0.18 0.36 0.26 9.6 9.6
28 10.00 30.0 15.0 20 16.0 5 0.36 0.48 0.39 0.41 6.8 7.2
29 10.00 15.0 35.0 10 16.0 3 0.68 0.80 0.65 0.87 15.1 16.2
30 10.00 30.0 15.0 20 25.0 3 0.74 0.68 0.73 0.59 7.7 7.4
31 11.50 15.0 35.0 10 25.0 5 0.07 −0.02 0.06 0.13 12.3 11.9
32 11.50 30.0 15.0 10 16.0 3 0.93 0.65 0.89 0.85 13.1 13.3
33 10.75 22.5 25.0 23 20.5 4 0.67 0.53 1.42 1.12 6.8 5.7
34 10.00 15.0 15.0 10 25.0 3 0.55 0.38 0.71 0.63 12.1 11.9
35 10.75 22.5 25.0 15 20.5 2 1.21 1.08 1.89 1.54 11.5 12.1
36 11.50 30.0 15.0 20 16.0 3 0.87 0.88 1.00 1.12 7.1 7.0
37 11.92 22.5 25.0 15 20.5 4 0.29 0.33 0.58 0.56 10.9 9.8
38 10.00 15.0 35.0 10 25.0 3 0.66 0.73 0.69 0.90 15.3 15.6
39 10.00 15.0 35.0 10 25.0 5 0.36 0.34 0.34 0.32 12.3 12.3
40 11.50 15.0 35.0 10 25.0 3 0.36 0.34 0.84 0.84 14.4 14.6
41 10.00 15.0 35.0 10 16.0 5 0.42 0.39 0.37 0.38 13.8 13.2
42 10.75 22.5 25.0 15 20.5 4 0.75 0.73 1.21 1.11 10.4 10.4
43 10.75 22.5 25.0 15 20.5 4 0.81 0.73 0.91 1.11 10.6 10.4
44 10.00 15.0 35.0 20 25.0 5 0.10 0.18 0.27 0.41 7.5 6.9
45 10.00 15.0 15.0 20 16.0 5 0.11 0.25 0.11 0.33 6.7 6.5
46 11.50 15.0 15.0 10 16.0 5 0.26 0.26 0.52 0.37 10.4 10.3
47 10.75 22.5 25.0 15 20.5 4 0.71 0.73 0.70 1.11 10.9 10.4
48 11.50 30.0 15.0 10 16.0 5 0.34 0.30 0.70 0.77 12.2 11.7
49 11.50 15.0 35.0 10 16.0 3 0.36 0.33 0.84 0.78 15.5 14.8
50 11.50 15.0 15.0 20 25.0 5 0.11 0.25 0.41 0.49 5.5 6.3
51 11.50 15.0 15.0 10 25.0 5 0.22 0.15 0.33 0.26 9.3 9.4
52 11.50 30.0 35.0 20 25.0 5 0.30 0.30 0.64 0.79 7.5 7.5
53 10.75 22.5 25.0 15 27.5 4 0.65 0.68 0.88 0.83 10.0 10.0
54 11.50 15.0 15.0 10 25.0 3 0.22 0.32 0.61 0.77 11.0 11.2
55 11.50 15.0 15.0 20 25.0 3 0.17 0.44 0.61 1.13 6.1 6.7
56 10.00 15.0 35.0 20 16.0 5 0.26 0.30 0.43 0.38 7.4 7.1
57 10.00 30.0 15.0 10 25.0 3 0.66 0.81 0.16 0.44 12.3 13.7
58 11.50 15.0 35.0 10 16.0 5 0.00 −0.05 0.05 0.16 11.0 12.4
59 11.50 15.0 35.0 20 16.0 5 0.00 0.15 0.38 0.37 6.7 6.9
60 11.50 30.0 15.0 10 25.0 5 0.02 0.30 0.38 0.72 9.9 10.7
61 10.00 30.0 15.0 10 16.0 5 0.47 0.56 0.52 0.49 11.3 12.6
62 10.00 15.0 15.0 10 16.0 3 0.53 0.60 0.56 0.68 12.7 12.9
63 11.50 30.0 35.0 10 16.0 3 0.02 0.50 0.90 0.76 16.1 16.7
64 10.00 30.0 15.0 20 16.0 3 0.81 0.86 0.56 0.50 8.2 7.9
65 10.00 30.0 15.0 10 16.0 3 0.83 0.93 0.36 0.44 14.2 14.8
66 10.00 15.0 15.0 10 16.0 5 0.51 0.38 0.51 0.40 11.1 10.8
67 11.50 15.0 15.0 20 16.0 5 0.70 0.43 0.56 0.51 5.8 6.6
68 10.75 22.5 25.0 15 20.5 4 0.68 0.73 0.99 1.11 9.6 10.4
69 11.50 30.0 35.0 20 16.0 3 0.94 0.77 0.89 1.10 8.0 8.6
70 10.00 30.0 15.0 20 25.0 5 0.28 0.31 0.13 0.41 6.1 6.5
71 10.00 30.0 35.0 20 25.0 5 0.33 0.50 0.15 0.46 7.2 7.8
72 10.75 34.2 25.0 15 20.5 4 0.82 0.80 0.78 1.05 10.1 11.3
73 11.50 30.0 35.0 20 16.0 5 0.10 0.22 0.70 0.67 7.5 7.5
74 10.75 22.5 25.0 15 20.5 6 0.16 0.33 0.40 0.75 8.4 8.7
75 10.00 15.0 15.0 20 16.0 3 0.56 0.48 0.92 0.75 7.8 7.2
76 10.00 15.0 35.0 20 25.0 3 0.54 0.58 1.01 1.12 8.0 8.7
77 10.75 22.5 25.0 15 16.0 4 0.65 0.77 0.84 0.97 10.4 10.6
78 10.75 22.5 25.0 15 20.5 4 0.64 0.73 1.00 1.11 9.4 10.4
79 9.58 22.5 25.0 15 20.5 4 0.57 0.60 0.20 0.22 10.1 10.9
80 11.50 30.0 35.0 20 25.0 3 0.76 0.82 1.25 1.30 8.3 8.9
81 11.50 15.0 35.0 20 16.0 3 0.54 0.55 0.96 1.13 8.1 8.0
82 10.00 30.0 35.0 20 25.0 3 0.85 1.05 0.64 0.84 9.8 9.7
83 10.75 22.5 25.0 15 20.5 4 0.73 0.73 1.10 1.11 10.3 10.4
84 10.75 22.5 25.0 7 20.5 4 0.27 0.47 0.54 0.84 16.1 15.1
85 11.50 30.0 35.0 10 25.0 3 0.86 0.62 0.92 0.88 16.0 16.3
86 10.00 15.0 35.0 20 16.0 3 0.83 0.72 1.10 1.00 8.6 8.7
c

Migration time of the last peak.