Table 6.

ANOVA table for models used in the optimization of MEKC, sheath liquid, and spray chamber parameters.

Responses	Source	Sum of squares	Degrees of freedom	Mean square	F-ratio	Prob > F
MEKC parameters
Resolution-BNP	Model	6.40	27	0.24	8.12	<0.0001
	Residual (error)	1.69	58	0.029
	Corrected total	8.09	85
Resolution-BOH	Model	9.07	27	0.34	4.97	<0.0001
	Residual (error)	3.92	58	0.068
	Corrected total	12.98	85
Migration time	Model	804.96	21	38.33	48.31	<0.0001
	Residual (error)	50.78	64	0.79
	Corrected total	855.73	85
Sheath liquid parameters
Peak area-BNP	Model	3.03 × 1010	9	3.38 × 109	2.84	0.0597
	Residual (error)	1.19 × 1010	10	1.19 × 109
	Corrected total	4.22 × 1010	19
Peak area-BOH	Model	2.42 × 1011	9	2.69 × 1010	5.48	0.0069
	Residual (error)	4.90 × 1010	10	4.90 × 109
	Corrected total	2.91 × 1011	19
S/N-BNP	Model	5.46 × 104	9	6.07 × 103	2.91	0.0557
	Residual (error)	2.09 × 104	10	2.09 × 103
	Corrected total	7.55 × 104	19
S/N-BOH	Model	1.41 × 106	9	1.57 × 105	3.80	0.0245
	Residual (error)	4.13 × 105	10	4.13 × 104
	Corrected total	1.82 × 106	19
Spray chamber parameters
Peak area-BNP	Model	9.41 × 108	3	3.14 × 108	0.76	0.5423
	Residual (error)	3.70 × 109	9	4.11 × 108
	Corrected total	4.64 × 1012	12
Peak area-BOH	Model	1.25 × 1010	2	6.23 × 109	1.63	0.2443
	Residual (error)	3.83 × 1010	10	3.83 × 109
	Corrected total	5.08 × 1010	12
S/N-BNP	Model	47.69	5	9.54	1.62	0.2709
	Residual (error)	41.22	7	5.89
	Corrected total	88.91	12
S/N-BOH	Model	7.71 × 102	5	1.54 × 102	3.74	0.0575
	Residual (error)	2.89 × 102	7	41.25
	Corrected total	1.06 × 103	12