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. Author manuscript; available in PMC: 2010 Apr 30.
Published in final edited form as: ACS Chem Biol. 2009 Nov 20;4(11):939–947. doi: 10.1021/cb900195c

Figure 6.

Figure 6

DFP analogs fail to inhibit HIV-1 replication in Jurkat cells. (a) HIV-1 replication is Nef-independent in Jurkat T-cells. Cells were infected in 96-well plates (200 µl final volume) with wild-type HIV strain NL4-3 (WT) or a mutant that fails to express Nef (ΔNef) over the range of viral titers shown. Relative HIV p24 levels were determined by ELISA 5 days later. (b) Jurkat cells were infected with equal titers of wild-type HIV (WT) or the Nef-defective mutant (ΔNef) in the presence of 5 µM of the DFP analogs (Figure 3). HIV p24 release was determined by ELISA 5 days later. Data are presented as percent of p24 release observed in the absence of compound ± S.D.