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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Cancer Res. 2010 Apr 13;70(9):3557–3565. doi: 10.1158/0008-5472.CAN-09-4674

Figure 3. Over-expression of CSF1 in human GBM.

Figure 3

(A) Immunohistochemical staining showing CSF1 (Arrows, right) and CSF1R (Arrows, left) staining in human GBM tissue sections. (B) Western blot of whole cell lysates from normal (post-mortem) brain (N1-N5) as well as from primary human GBMs (T1-T15). Bars represent the ratio of CSF1 to GAPDH signal intensity. (C) qRT-PCR result showing elevated levels of CSF1 transcript in normal (n=5) vs. GBM (n=18) tissue (p-value generated using Wilcoxon rank-sum test). (D) Top: Dual label immunofluorescence images illustrating tumor cells (white arrows) expressing both CSF1 (Red) and the glial marker GFAP (Green). Bottom: Separate immunofluorescence experiment illustrating distinct CSF1 staining in (red) tumor cells (arrowheads) and CD45 staining in (green) infiltrating lymphoid/myeloid cells.