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. Author manuscript; available in PMC: 2011 Jun 5.
Published in final edited form as: Virology. 2010 Mar 12;401(2):146–154. doi: 10.1016/j.virol.2010.02.004

Fig 3. Actin reorganization following TAstV-2 infection.

Fig 3

(A) Control and TAstV-2-infected jejunum sections collected at 4 dpi were stained for F-actin using phalloidin conjugated with Alexa fluor 546 (red), TAstV-2 nonstructural protein (green) and nuclei (blue), as detailed in methods. Arrow heads denote normal apical F-actin fluorescence, while arrows denote areas of rearrangement in actin. F-actin reorganization was prominent in the areas of virus localization (yellow color in merged panel). White bar represents 50µm. (B) Fluorescence images from TAstV-2 infected tissues were overlaid with a 25 µm grid. Individual grids were scored for actin rearrangement (normal/comparable with control tissues, moderate changes, severe changes). Grids were then assessed for the presence or absence of astrovirus fluorescence within the infected epithelium. Three villi, from 3 different samples were analyzed. The percent distribution of both parameters was calculated and means represented by bars. * denotes significant difference p < 0.001 between changes in actin in grids with TAstV-2 and grids without.