Fig. 2.

A C-terminal ICP27 mutant does not colocalize with SRp20. HeLa cells were transfected with GFP-SRp20 and were subsequently infected with KOS, d4–5 or C-terminal mutant n406. Cells were fixed at 5 h after infection and stained with antibody directed to ICP27. GFP fluorescence was visualized directly. Images were viewed with an LSM 510 confocal microscope at 63X. Z-stack analysis was performed on merged images and histograms showing the patterns of green pixels (ICP27) compared to blue pixels (SRp20) in the Z-stack layers are shown to the right.