Figure 3.
In vivo response of nanosensors to glucose. (A) Image of mouse injected at four locations with glucose-sensitive nanosensors. Image was obtained with an IVIS®-Spectrum imaging system. Excitation and emission wavelengths were 500 nm and 600 nm, respectively. Intensity bar displays the normalized fluorescence efficiency, which represents the fractional ratio of fluorescent emitted photons per incident excitation photon. Residual background fluorescence was attributed to remaining fur. (B) The representative response to oral gavage of the blood glucose (red) and fluorescence of the glucose nanosensors (black). Mean ± SD for one mouse is shown. (C) Correlation in vivo between optical response of nanosensors and blood measurements. Glucose concentration from optical data vs. blood glucose is shown for all mice (n = 4). Error bars are omitted for clarity. A line was fitted to the plot (slope = 1.0, R2 = 0.75).