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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Dev Biol. 2010 Mar 11;341(2):444–458. doi: 10.1016/j.ydbio.2010.03.001

Fig. 4.

Fig. 4

Chl and CHL1 are substrates for BMP1/Tolloid-like proteinases. (A) A Coomassie stained SDS-PAGE gel is shown of protein molecular weight markers (lane 1) and of ~55-kDa secreted and purified Chl (lane 2). (B–D) Immunoblotting with anti-FLAG antibody (B and D) or anti-CHL1 antibody (C) is shown for in vitro assays in which recombinant Chl was incubated in the absence (−) or presence (+) of Bmp1a (B), or in which recombinant short, neuralin form of CHL1 (C) or CHL2 (D) was incubated in the absence (o/n) or presence of mammalian BMP1, mTLD, mTLL1, or mTLL2. Positions of the ~30-kDa Chl (B) and ~20-kDa and smaller CHL1 (C) cleavage products are shown. In B and D, asterisks mark the positions of FLAG-tagged BMP1 and related proteinases. Molecular masses (in kDa) are indicated for protein standards.