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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Dev Biol. 2010 Mar 11;341(2):444–458. doi: 10.1016/j.ydbio.2010.03.001

Fig. 8.

Fig. 8

Synergistic effects of Chl and Chd MOs in inducing ventralization does not involve p53-mediated off target effects. (A) A comparison is shown of 24 hpf embryos treated with 0.38 mM StCon-MO, 0.1 mM Chl 5′1-MO, 0.026 mM Chd-MO plus 0.25 mM p53-MO, 0.1 mM Chl 5′1-MO plus 0.026 mM Chd-MO, or with 0.1 mM Chl 5′1 plus 0.026 mM Chd-MO and 0.25 mM p53-MO. (B) Quantification of phenotypes at 24 hpf. Severity of dorsalization (D) is numerically classified as per Mullins et al. (Mullins et al., 1996). Degree of ventralization (VE) was not numerically classified. (C–D) Whole mount in situ hybridization is shown for expression of the dorsal marker Chl at 30% epiboly for embryos treated with 0.38 mM StCon-MO (C), 0.1 mM Chl 5′1-MO plus 0.25 mM p53-MO (D), 0.026 mM Chd-MO plus 0.25 mM p53-MO (E), or 0.1 mM Chl 5′1 plus 0.026 mM Chd-MO and 0.25 mM p53-MO.