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. Author manuscript; available in PMC: 2011 May 7.
Published in final edited form as: Vaccine. 2010 Mar 21;28(21):3696–3705. doi: 10.1016/j.vaccine.2010.03.016

Figure 5. Cytokine production in BMDC induced by treatment with LT-IIa-B5.

Figure 5

BMDC (5×106) derived from wt C57BL/6 mice (WT) or C57Bl/6 TLR2-/- mice (TLR2-/-) were incubated for 6 hrs at 37°C with PBS (Untreated), 100 ng/ml of LPS, or with 5 μg/ml of LT-IIa-B5. Total RNAs isolated from the BMDC were analyzed by qRT-PCR for mRNA encoding IL-12p40, TNF-α, IL-10, IL-4, and IFN-γ. Data are presented as fold change in expression of the cytokine from expression of that cytokine in the untreated BMDC. Error bars denote one standard deviation from the mean obtained from triplicate cultures. Key: +, TLR2-proficient BMDC; -, TLR2-deficient BMDC; white bars, BMDC treated with LPS; black bars, BMDC treated with LT-IIa-B5; *, statistical difference from the untreated control (P<0.05).