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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Cancer Res. 2010 Apr 13;70(9):3803–3812. doi: 10.1158/0008-5472.CAN-09-2648

Figure 1. Inducible expression of activated Ras in primary endothelial cells.

Figure 1

(A) pSLIK-RasV12-Venus lentiviral vectors were used to obtain HUVECs stably expressing activated Ras (B) infection efficiencies are shown by the histograms for Venus expression. (C) Cells were induced with doxycycline and Ras expression was measured. (D) Serum starved HUVECs were induced with 50 ng/ml doxycycline and whole cell lysates were probed as indicated. Data are representative of three independent infections on independent lots of endothelial cells.