Fig. 2. STAT3^Mye−/− mice have more neutrophil infiltration in the liver than wild-type mice after CCl4 treatment.

Wild-type (WT) and STAT3^Mye−/− mice were injected with CCl₄ and sacrificed 24 h post injection. A. Liver tissues were stained with anti-MPO (myeloperoxidase) antibody for neutrophils (MPO positive brown staining). B. Liver immune cells were isolated and analyzed by flow cytometry with anti-CD11b and anti-Gr-1 antibodies. Gr-1^brightCD11b⁺ cells in the R3 area represent neutrophils. C, D. The percentage and the total number of liver lymphocytes were quantified. Values represent means±SD (n=4–5). *P<0.05, in comparison with corresponding littermate WT groups.