Fig. 4. Elevated hepatic STAT3 activation in STAT3^Mye−/− mice.

A,B: Mice were injected with CCl₄ and then sacrificed at various time points. Liver tissues were collected for Western blot analyses (A) or for measurement of GSH levels and NHE-His adduct (B). C, D: Kupffer cells were isolated from wild-type and STAT3^Mye−/− mice, and treated with LPS (1ng/ml) for 24 hours. The supernatants were collected for measurement of various cytokines (C), and cells were collected for real-time PCR analyses (D). Values represent means±SD (n=5). *P<0.05 in comparison with corresponding WT littermate groups. E, STAT3^Mye−/− mice were treated with control Ab or anti-IL-6 antibody for 2 days. Liver tissues were then collected for Western blot analyses.