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. Author manuscript; available in PMC: 2011 Apr 30.
Published in final edited form as: Circ Res. 2010 Mar 11;106(8):1413–1424. doi: 10.1161/CIRCRESAHA.109.209312

Figure 6.

Figure 6

Spectroscopic determination of luminal [Ca2+] of the SR in saponin-permeabilized WT and R2474S/+ KI cardiomyocytes (see “Expanded Materials and Methods” in online data supplements for measurement of intra-SR [Ca2+]). (Left) Representative fluo-5N images before and after addition of caffeine (20 mmol/L). (Right) Summarized data of the luminal [Ca2+] normalized to control condition of WT cardiomyocytes. N: the number of cells from 3–4 hearts.