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. 2010 May 5;98(9):1820–1829. doi: 10.1016/j.bpj.2010.01.010

Figure 1.

Figure 1

Typical behavior of OmpU and OmpT at neutral and acidic pHs. Representative patch-clamp recordings of a (A and B) single OmpT trimer and (C–E) a single OmpU trimer in symmetric conditions of 150 mM KCl in buffer at the indicated pHs. The pipette voltage is +50 mV. The tick mark labeled “open” indicates the current level of the fully open trimer; C1, C2, and C3 indicate the levels corresponding to the closures OmpT monomers (A and B). (C–E) Tick mark of labeled SSC indicate the single step closure level observed for OmpU. The dashed line is the 0 pA level. Note the increase in the size of the SSC whereas the full conductance of the open trimer does not vary.