Figure 2.

Sedimentation coefficient distributions c(s) representing the boundary patterns of the interacting system of Fig. 1 at different total loading concentrations. The vertical lines indicate the s values of the free and complex species. (Top) Dilution series with equimolar concentrations at 0.1 K_D (blue), 0.3 K_D (pink), K_D (green), 3 K_D (red), and 10 K_D (cyan). The c(s) distributions are normalized relative to the total loading concentrations. (Bottom) Titration series of a constant total concentration c_Atot= K_D of the smaller species A with increasing concentrations c_Btot of 0.1 K_D (blue), 0.3 K_D (pink), K_D (green), 2.366 K_D (red), and 10 K_D (cyan). Distributions are not normalized. For both panels, sedimentation and reaction parameters are as in Fig. 1, with signal coefficients of 40,000 M⁻¹ cm⁻¹ and 60,000 M⁻¹ cm⁻¹ for A and B, respectively.