Table 1.
Experimental parameters for cell-division events in the absence and presence of AIs
| Sample | [AI] (nM) | M | F0 (count) | σA | σN | N0 (copy) |
|---|---|---|---|---|---|---|
| 1 | 0 | 230 | 12559 ± 2794 | 3.39% ± 0.13% | 5.64% ± 0.20% | 79 ± 5 |
| 2 | 0 | 256 | 19133 ± 3693 | 3.49% ± 0.10% | 4.80% ± 0.12% | 108 ± 5 |
| 3 | 0 | 178 | 23916 ± 5682 | 3.19% ± 0.15% | 4.30% ± 0.20% | 135 ± 12 |
| 4 | 10 | 292 | 27485 ± 4371 | 3.94% ± 0.25% | 3.84% ± 0.20% | 169 ± 18 |
| 5 | 18 | 156 | 33726 ± 7123 | 3.15% ± 0.20% | 3.75% ± 0.22% | 178 ± 21 |
| 6 | 22.5 | 264 | 16902∗ ± 2589 | 3.97% ± 0.18% | 360∗ ± 55 |
AI is the exogenous concentration of AI-1 and AI-2 during growth of the colony. M is the total number of division events in each sample. F0 is the ensemble-averaged peak fluorescence immediately before cell division. σA and σN are the standard deviations of PA(x) and P(y/x), respectively, inferred by MLE (see text). N0 is the LuxR dimer number immediately before cell division inferred by MLE (in Samples 1–5). In Sample 6, the incident power was reduced significantly to avoid phototoxicity arising from the enhanced photon absorption by the much higher concentration of LuxR-mCherry (incident powers are identical for Samples 1–5). In Sample 6, the value of σN was too small to be reliably obtained by MLE. In this case, values of N0 are inferred from F0 using the scaling constant ν established in Fig. 4C.