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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Nat Genet. 2002 Feb 4;30(4):401–405. doi: 10.1038/ng838

Fig. 3.

Fig. 3

Transgenic rescue of hearing impairment in B6-tub/tub by the 129P2/OlaHsd allele of Mtap1a. a, Transgenic rescue construct. Primers for amplification of the GCTCAA repeat used to distinguish between the B6 allele and the transgene derived from 129P2/OlaHsd alleles are indicated. b, Detection of transgene-positive (Tg+) mice by PCR. The size of the resultant PCR products are 274 bp for 129P2/OlaHsd and 214 bp for B6. c, Expression of the transgene-derived Mtap1a in the cochlea as detected by RT–PCR with the primers used for genotyping (the third band which appears under the transgene-derived 274 bp band represents the heteroduplex between the B6 and 129P2/OlaHsd alleles). d, ABR thresholds in C57BL/6-tub/tub-Tg+ mice. The mean ± s.e.m. of the sound pressure threshold is shown for each stimulus and genotype. Five or six mice at 5–6 wk were tested for each genotype. Threshold levels with different superscripts are significantly different (P<0.01).