Table 3.
MtDNA sequence variation between the three inbred mouse strains and the published mouse sequence
| Position (bp) | Published sequence | A/J | NOD/LtJ | SKH2/J | Gene/RNA | Amino acid substitution |
|---|---|---|---|---|---|---|
| 1767 | A | G | G | G | mt-Rnr2 | |
| 3815 | T | del T | del T | del T | mt-Tg | |
| 3824 | ins T | ins T | ins T | mt-Tg | ||
| 4012 | A | G | G | G | mt-Nd2 | none |
| 4794 | T | C | C | C | mt-Nd2 | Ile→Thr |
| 5734 | C | T | T | T | mt-Co1 | Pro→Leu |
| 5737 | T | C | C | C | mt-Co1 | Val→Ala |
| 6238 | G | A | A | A | mt-Co1 | Cys→Tyr |
| 9348 | A | A | A | G | mt-Co2 | Ile→Val |
| 9553 | ins AAA | ins AAA | ins AAA | mt-Nd3 | ||
| 9826 | ins A | ins A | ins A | mt-Tr | ||
| 9827 | ins A | mt-Tr | ||||
| 9856 | C | A | A | A | mt-Tr | |
| 10059 | ins CAT | ins CAT | ins CAT | mt-Nd4 | ||
| 12042 | C | T | T | T | mt-Nd5 | Leu→Phe |
| 15823 | T | delT | delT | delT | D-loop | |
| 16119 | A | C | C | C | D-loop | |
| 16241 | A | delA | delA | delA | D-loop |
A total of 18 changes from the originally published mouse mtDNA sequence were identified15. All appeared homoplasmic within the level of detection of the sequencing gels. Sixteen of the changes were found in all three mouse strains. Fifteen of them, with the exception of the insertion of an adenine at position 9826 in mt-Tr, represent most likely errors in the published sequence. A BLAST search of the mouse EST database and non-redundant database revealed that all available ESTs, as well as two previously sequenced mouse strains, MilP and NZB/B1NJ (ref. 30), contained the same sequence changes. The A9348G sequence change was found only in the SKH2/J mouse. It appears to be a polymorphism, as the MilP and NZB/B1NJ strains, as well as 40 of the mouse ESTs, contained a guanine, whereas nine ESTs contained an adenine.